Thermo Fisher Scientific is hiring a Scientist II, qPCR Molecular Biologist to join our R&D team. You will make a meaningful impact on global health by developing next-generation sequencing technologies, diagnostic assays, and molecular biology products that help our customers make breakthrough discoveries.
What You'll Do
- Design, develop, and optimize qPCR assays for gene expression, genotyping, copy number variation, and pathogen detection.
- Plan and execute experiments independently, ensuring data quality, reproducibility, and technical excellence.
- Perform nucleic acid extraction, cDNA synthesis, and quantification using standard molecular methods.
- Validate assay performance (sensitivity, specificity, and efficiency) and analyze results using platforms such as Applied Biosystems QuantStudio™ or Bio-Rad CFX Manager.
- Troubleshoot technical issues and implement improvements to enhance assay performance.
- Maintain detailed experimental records and prepare technical reports, SOPs, and validation documentation.
- Collaborate with product development, bioinformatics, and assay design teams to integrate molecular workflows.
- Ensure adherence to safety protocols, quality systems, and regulatory requirements (ISO, GLP, CLIA).
What We're Looking For
- Bachelor’s or Master’s in Molecular Biology, Biochemistry, Genetics, or a related life sciences discipline.
- Minimum of 1-4 years of hands-on experience performing qPCR.
- Prior experience with assay development, validation, and data analysis.
- Understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting.
- Strong analytical and problem-solving skills with a data-driven mindset.
- Strong understanding of qPCR principles, primer/probe design, and amplification kinetics.
- Proficiency in data analysis, statistical interpretation, and report generation.
- Excellent organizational, communication, and problem-solving skills.
- Ability to work independently as well as collaboratively in a multidisciplinary environment.
- Strong attention to detail and scientific curiosity.
Nice to Have
- Familiarity with nucleic acid extraction instruments.
- Experience working under quality and regulatory standards.
- Familiarity with bioinformatics tools for assay design.
- Experience with NGS.
- Experience with digital PCR (dPCR).
- Experience with qPCR multiplexing.
- Knowledge of automation and data management systems.
Technical Stack
- qPCR, Applied Biosystems QuantStudio™, Bio-Rad CFX Manager
- Nucleic acid extraction, cDNA synthesis
- Next-generation sequencing (NGS), digital PCR (dPCR)
Team & Environment
This role involves collaboration within cross-functional teams, including product development, bioinformatics, and assay design.
Work Mode
This position is onsite.
Thermo Fisher Scientific is an equal opportunity employer.
